WORLD JOURNAL OF PLASTIC SURGERY
Thu, Apr 25, 2024
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Volume 7, Issue 2 (2018)
WJPS 2018, 7(2): 204-211 |
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Hashemi S, Jowkar S, Mahmoodi M, Rafati A R, Mehrabani D, Zarei M et al . Biochemical Methods in Production of Three-Dimensional Scaffolds from Human Skin: A Window in Aesthetic Surgery. WJPS 2018; 7 (2) :204-211
URL: http://wjps.ir/article-1-411-en.html
URL: http://wjps.ir/article-1-411-en.html
Seyede-Sara Hashemi1 
, Somayeh Jowkar2 , Mahdokht Mahmoodi3 , Ali Reza Rafati4 , Davood Mehrabani3 , Masoumeh Zarei5 , Abdolkhalegh Keshavarzi 6
, Somayeh Jowkar2 , Mahdokht Mahmoodi3 , Ali Reza Rafati4 , Davood Mehrabani3 , Masoumeh Zarei5 , Abdolkhalegh Keshavarzi 6
1- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
2- Department of Biology, Center of Taft, Payame Noor University, Taft, Iran
3- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran
5- Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran
6- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Rohan Gene Cell Tech, Shiraz, Iran , keshvarzg@sums.ac.ir
2- Department of Biology, Center of Taft, Payame Noor University, Taft, Iran
3- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran
5- Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran
6- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Rohan Gene Cell Tech, Shiraz, Iran , keshvarzg@sums.ac.ir
Abstract: (4582 Views)
BACKGROUND
Use of matrix-derived biologic scaffolds has become a treatment of choice in several clinical issues. This study assessed biochemical methods in production of three-dimensional scaffolds from human skin.
METHODS
Human skin was prepared from circumcisions, washed in phosphate buffer saline (PBS) and kept at -20ºC until use. The skin samples underwent various methods. In group A, NaCl, Triton X100 and EDTA solution were used for removal of epidermis and was subdivided to three subgroups. The solution for removal of epidermis was similar for all subgroups, but decellularization was different. Group B was subdivided into 6 subgroups, NaCl in different concentrations was used for removal of epidermis and decellularization happened using SDS in various concentrations and different time intervals. Group C was subdivided to 3 subgroups, trypsin was used for removal of epidermis and decellularization was conducted applying NaOH or SDS. Washing was performed using only PBS. In group D, decellularization was done applying SDS. Histomorphometric study was conducted to compare the groups.
RESULTS
No fibroblast was present in A2, B2, B4, and C3 subgroups after decellularization. Histological photographs from subgroups A1 to A3 revealed several cells and collagen fibers. Dense collagen fibers in pink color were noted in all subgroups; but, epidermis was absent.
CONCLUSION
It was shown that 1M NaCl was the best solution for removal of epidermis, 0.5% SDS for 2 h was the most effective solution for decellularization and PBS was the best solution for washing, while the solutions are easily available and cost-effective.
Use of matrix-derived biologic scaffolds has become a treatment of choice in several clinical issues. This study assessed biochemical methods in production of three-dimensional scaffolds from human skin.
METHODS
Human skin was prepared from circumcisions, washed in phosphate buffer saline (PBS) and kept at -20ºC until use. The skin samples underwent various methods. In group A, NaCl, Triton X100 and EDTA solution were used for removal of epidermis and was subdivided to three subgroups. The solution for removal of epidermis was similar for all subgroups, but decellularization was different. Group B was subdivided into 6 subgroups, NaCl in different concentrations was used for removal of epidermis and decellularization happened using SDS in various concentrations and different time intervals. Group C was subdivided to 3 subgroups, trypsin was used for removal of epidermis and decellularization was conducted applying NaOH or SDS. Washing was performed using only PBS. In group D, decellularization was done applying SDS. Histomorphometric study was conducted to compare the groups.
RESULTS
No fibroblast was present in A2, B2, B4, and C3 subgroups after decellularization. Histological photographs from subgroups A1 to A3 revealed several cells and collagen fibers. Dense collagen fibers in pink color were noted in all subgroups; but, epidermis was absent.
CONCLUSION
It was shown that 1M NaCl was the best solution for removal of epidermis, 0.5% SDS for 2 h was the most effective solution for decellularization and PBS was the best solution for washing, while the solutions are easily available and cost-effective.
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