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:: Volume 7, Issue 2 (4-2018) ::
wjps 2018, 7(2): 204-211 Back to browse issues page
Biochemical Methods in Production of Three-Dimensional Scaffolds from Human Skin: A Window in Aesthetic Surgery
Seyede-Sara Hashemi , Somayeh Jowkar , Mahdokht Mahmoodi , Ali Reza Rafati , Davood Mehrabani , Masoumeh Zarei , Abdolkhalegh Keshavarzi
Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Rohan Gene Cell Tech, Shiraz, Iran
Abstract:   (643 Views)
BACKGROUND
Use of matrix-derived biologic scaffolds has become a treatment of choice in several clinical issues. This study assessed biochemical methods in production of three-dimensional scaffolds from human skin.
METHODS
Human skin was prepared from circumcisions, washed in phosphate buffer saline (PBS) and kept at -20ºC until use. The skin samples underwent various methods. In group A, NaCl, Triton X100 and EDTA solution were used for removal of epidermis and was subdivided to three subgroups. The solution for removal of epidermis was similar for all subgroups, but decellularization was different. Group B was subdivided into 6 subgroups, NaCl in different concentrations was used for removal of epidermis and decellularization happened using SDS in various concentrations and different time intervals. Group C was subdivided to 3 subgroups, trypsin was used for removal of epidermis and decellularization was conducted applying NaOH or SDS. Washing was performed using only PBS. In group D, decellularization was done applying SDS. Histomorphometric study was conducted to compare the groups.
RESULTS
No fibroblast was present in A2, B2, B4, and C3 subgroups after decellularization. Histological photographs from subgroups A1 to A3 revealed several cells and collagen fibers. Dense collagen fibers in pink color were noted in all subgroups; but, epidermis was absent.
CONCLUSION
It was shown that 1M NaCl was the best solution for removal of epidermis, 0.5% SDS for 2 h was the most effective solution for decellularization and PBS was the best solution for washing, while the solutions are easily available and cost-effective.
Keywords: Scaffold, ADM, Human skin, Aesthetic medicine
Full-Text [PDF 3754 kb]   (192 Downloads)    
Type of Study: Original Article | Subject: General
Received: 2018/04/27 | Accepted: 2018/04/27 | Published: 2018/04/27
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Hashemi S, Jowkar S, Mahmoodi M, Rafati A R, Mehrabani D, Zarei M et al . Biochemical Methods in Production of Three-Dimensional Scaffolds from Human Skin: A Window in Aesthetic Surgery. wjps. 2018; 7 (2) :204-211
URL: http://wjps.ir/article-1-411-en.html


Volume 7, Issue 2 (4-2018) Back to browse issues page
مجله جهانی جراحی پلاستیک World Journal of Plastic Surgery
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