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:: Volume 6, Number 2 (4-2017) ::
3 2017, 6(2): 198-205 Back to browse issues page
The Role of Human Adult Peripheral and Umbilical Cord Blood Platelet-Rich Plasma on Proliferation and Migration of Human Skin Fibroblasts
Seyedeh-Sara Hashemi, Mahdokht Mahmoodi, Ali Reza Rafati , Davood Mehrabani
Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran
Abstract:   (689 Views)

BACKGROUND

Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts.

METHODS

Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilical cord blood and adult peripheral blood were provided and added to fibroblasts cultured from a human skin sample. Migration and proliferation of fibroblasts were assessed in comparison to 10% FBS and by the fibroblast responses to a concentration gradient.

RESULTS

All components of the umbilical cord blood PRP significantly stimulated the growth of fibroblasts when compared to the negative control. Fibroblast growth was enhanced in a dose dependent manner. All fibroblast cultures retained normal morphology. No significant difference was noted between umbilical cord blood and adult peripheral blood PRP preparations regarding cell proliferation and migration, but the difference to 10% FBS was significant. 1% and 50% PRP reduced cellular proliferation. The 20% umbilical cord blood PRP and 10% adult peripheral blood PRP had a significant stimulatory effect on the migration of the skin fibroblast cells in comparison with 10% FBS.

CONCLUSION

As PRP could promote the migration and proliferation of dermal fibroblasts, it can be safely added in cultures when treatment of chronic wounds without triggering the immune response is needed.

Keywords: Umbilical cord blood, Platelet rich plasma, Proliferation, Migration, Skin fibroblast
Full-Text [PDF 1016 kb]   (415 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2017/02/18 | Accepted: 2017/02/18 | Published: 2017/02/18
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Hashemi S, Mahmoodi M, Rafati A R, Mehrabani D. The Role of Human Adult Peripheral and Umbilical Cord Blood Platelet-Rich Plasma on Proliferation and Migration of Human Skin Fibroblasts. 3. 2017; 6 (2) :198-205
URL: http://wjps.ir/article-1-323-en.html
Volume 6, Number 2 (4-2017) Back to browse issues page
مجله جهانی جراحی پلاستیک World Journal of Plastic Surgery
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