دوره 9، شماره 3 - ( 6-1399 )                   جلد 9 شماره 3 صفحات 313-320 | برگشت به فهرست نسخه ها


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rasekh H, Hoseini Farahi M, Mehrabani D, Massoumi S J, Ramzi M, Acker J P. Proliferative and Regenerative Effect of Acetonic Extract of Feijoa sellowiana on Stem Cells. wjps. 2020; 9 (3) :313-320
URL: http://wjps.ir/article-1-593-fa.html
Proliferative and Regenerative Effect of Acetonic Extract of Feijoa sellowiana on Stem Cells. مجله جهانی جراحی پلاستیک. 1399; 9 (3) :313-320

URL: http://wjps.ir/article-1-593-fa.html


چکیده:   (771 مشاهده)
BACKGROUND
Feijoa is widely used in medicine due to their anti-inflammatory, antioxidant, antimicrobial and antitumor properties. The current investigation studied the proliferative and regenerative effect of acetonic extract of Feijoa sellowiana on stem cells.
METHODS
Acetone extract of Feijoa was prepared using percolator and rotary machines. Human bone marrow stem cells (hBMSCs) were used as experimental in vitro model and characterized morphologically, by flowcytometry, and differentiation properties. The toxicity of the extract on hBMSCs was determined by MTT assay. The viability and growth kinetics of hBMSCs treated to Feijoa was determined. Real time PCR was used for changes in expression of proliferative and apoptotic genes on day 7th.  
RESULTS 
MTT assay demondtrated that Feijoa at doses less than 200 ng/ml did not show any cytotoxic effect on hBMSCs and increased the cell proliferation until day 3rd followed by a non-significant slow decreasing trend until day 7th. Population doubling time (PDT) showed a decline until day 3rd followed by an increase until day 7th. A significant rise in expression of Bax and decline in Bcl-2 expression were noted on day 7th.
CONCLUSION 
The modulatory activity of Feijoa may be responsible for its increasing effect on cell proliferation till day 3rd. Therefore, when faster proliferation during a shorter time period is targeted, Feijoa can be safely added to the culture media in the first three days.
     
نوع مطالعه: مقالات اصيل | موضوع مقاله: عمومى
دریافت: 1398/11/24 | پذیرش: 1399/3/1 | انتشار: 1399/8/5

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
CAPTCHA

کلیه حقوق این وب سایت متعلق به مجله جهانی جراحی پلاستیک می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2021 All Rights Reserved | World Journal of Plastic Surgery

Designed & Developed by : Yektaweb