en تخصصي Special مقالات اصيل Original Article <span style="font-size:12pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:#0070c0"><b><span style="color:black">Background:</span></b><span style="color:black"> We aimed to determine the effect of <i>Zataria multiflora </i>Nanoemulsion gel on surgical wound healing.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">: This experimental study was conducted in the years 2021-2022 at the Animal Research Center of Mazandaran University of Medical Sciences, northern Iran. Forty two male Westar rats were randomly divided into six groups (n=7). After a surgical incision of full thickness with a 3 cm diameter, they were treated for 21 days with diltiazem 2% (positive control), placebo, and <i>Z. multiflora</i> emulsions and nanoemulsions at 2% and 4%, respectively. Macroscopic parameters of wound area and contraction, as well as pathological factors such as granulation, angiogenesis, epithelialization, collagen organization, bacterial colony, inflammation, creatine and epidermal thickness, hair follicles, and lymphatic ducts, were examined.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results</span></span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">: The mean wound size and contraction of the placebo group differed significantly from the other groups on all days, and on some days, the results indicated more favorable effects of nanoemulsions than Diltiazem. Based on the microscopic findings, the average scores on the seventh day were nearly different (<i>P</i>= 0.051); however, all groups scored higher than the placebo group. On the 21^st day, the best results were related to the 4% Nanoemulsion (242.5), 2% Nanoemulsion (159.4), and 4% emulsion (159.3), followed by diltiazem (154.60), 2% emulsion (146.5), and placebo (70.7).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion</span></span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">: <i>Z. multiflora</i> emulsions and nanoemulsions at 2% and 4% could be effective in healing surgical wounds, and the use of 4% Nanoemulsion yields the best results. This is recommended for use in clinical trial studies.</span></span></span></span></span></span></span><br> &nbsp; Medicinal herbs, Nanoemulsion, Wounds, Gel, Zataria multiflora 33 42 http://wjps.ir/browse.php?a_code=A-10-375-3&slc_lang=en&sid=1 Alireza Ganjipour 100319475328460016485 100319475328460016485 Yes Department of Anesthesiology, Operating room, School Allied medical Sciences,, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran Ebrahim Nasiri-Formi 100319475328460016486 100319475328460016486 No 3. Department of Anesthesiology, Operating room, School of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran Soheil Azizi 100319475328460016487 100319475328460016487 No 5. Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Jafar Akbari 100319475328460016488 100319475328460016488 No 6. Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Hooshang Akbari 100319475328460016489 100319475328460016489 No 3. Department of Anesthesiology, Operating room, School of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran Seyyed Mohammad Hassan Hashemi 100319475328460016490 100319475328460016490 No Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran