en عمومى General مقالات اصيل Original Article <strong>BACKGROUND</strong><br> Previous studies indicated that both <em>Plantago major</em> and <em>Aloe vera</em> have anti-inflammatory, tissue regeneration, antioxidant, and immune-stimulatory effects. It is assumed that a mixture of these two herbal medicines may provide a potent material in treatment of skin wound injuries. Therefore, in this study we investigated the effects of <em>Plantago major</em> and <em>Aloe vera</em> mixture in the process of wound healing in rat models according to stereological parameters.<br> <strong>METHODS</strong><br> In an experiential study, 36 male Sprague-Dawley rats (200&plusmn;20 g) were randomly assigned into three groups (n=12): The control group which received no treatment, gel base treated group, and the 5% <em>Plantago major</em> and 5% <em>Aloe vera</em> mixture gel treated group (PA group). Treatments were done every 24 hrs for 15 days. Wound closure rate, volume densities of the collagen bundles and the vessels, vessel&rsquo;s length density and mean diameter, and fibroblast populations were estimated using stereological methods.<br> <strong>RESULTS</strong><br> PA treated group showed faster wound closure rate in comparison with control and gel-base groups (<em>p</em><0.05). Numerical density of fibroblasts, volume density of collagen bundles, mean diameter, and volume densities of the vessels in PA group were significantly higher than the control and the gel-base treated groups (<em>p</em><0.05).<br> <strong>CONCLUSION</strong><br> We showed that <em>Plantago major</em> and <em>Aloe vera</em> mixture has the ability to improve wound healing by enhancing fibroblast proliferation, collagen bundle synthesis and re-vascularization in skin injuries. Wound healing, Fibroblast, Collagen, Vascularization, Plantago major, Aloevera, Stereology, Rat 51 57 http://wjps.ir/browse.php?a_code=A-10-142-1&slc_lang=en&sid=1 Soheil Ashkani-Esfahani 10031947532846006471 10031947532846006471 No Student Research Committee, Shiraz university of Medical Sciences, Shiraz, Iran Mahsima Khoshneviszadeh 10031947532846006472 10031947532846006472 No Medicinal and Natural products Chemistry Research Center, Shiraz university of Medical Sciences, Shiraz, Iran Ali Noorafshan 10031947532846006473 10031947532846006473 Yes Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Ramin Miri 10031947532846006474 10031947532846006474 No Medicinal and Natural products Chemistry Research Center, Shiraz university of Medical Sciences, Shiraz, Iran Shima Rafiee 10031947532846006475 10031947532846006475 No Student Research Committee, Shiraz university of Medical Sciences, Shiraz, Iran Kimia Hemyari 10031947532846006476 10031947532846006476 No Student Research Committee, International branch, Shiraz University of Medical Sciences, Shiraz, Iran Sina Kardeh 10031947532846006477 10031947532846006477 No Student Research Committee, Shiraz university of Medical Sciences, Shiraz, Iran Omid Koohi Hosseinabadi 10031947532846006478 10031947532846006478 No Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Dorna Fani 10031947532846006479 10031947532846006479 No Student Research Committee, Shiraz university of Medical Sciences, Shiraz, Iran Elnaz Faridi 10031947532846006480 10031947532846006480 No Student Research Committee, International branch, Shiraz University of Medical Sciences, Shiraz, Iran