en تخصصي Special مقالات اصيل Original Article <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><b><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">BACKGROUND</span></b></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult </span><span mso-bidi-language:="" new="" style="font-size:12.0pt; line-height:200%;font-family:" times="">peripheral </span><span new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts.</span></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><b><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">METHODS</span></b></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilical cord blood and adult </span><span new="" style="font-size:12.0pt;line-height: 200%;font-family:" times="">peripheral </span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">blood were provided and added to fibroblasts cultured from a </span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">human skin sample</span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">. Migration and proliferation of fibroblasts were assessed in comparison to 10% FBS and by the fibroblast responses to a concentration gradient.</span></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><b><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">RESULTS</span></b></p> <p class="MsoNormal" style="text-align:justify;line-height:200%;direction:ltr; unicode-bidi:embed"><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">All components of the umbilical </span><span new="" style="font-size:12.0pt;line-height:200%;font-family: " times="">cord</span><span lang="EN-CA" mso-bidi-language:="" new="" style="font-size:12.0pt;line-height:200%;font-family:" times=""> blood</span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%; font-family:" times=""> </span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">PRP significantly stimulated the growth of fibroblasts when compared to the negative control. Fibroblast growth was enhanced in a dose dependent manner. All fibroblast cultures retained normal morphology</span><span new="" style="font-size:12.0pt; line-height:200%;font-family:" times="">. No significant difference was noted between </span><span lang="EN-CA" mso-ansi-language:="" new="" style="font-size:12.0pt; line-height:200%;font-family:" times="">umbilical </span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">cord</span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height: 200%;font-family:" times=""> blood and</span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%;font-family:" times=""> </span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">adult </span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">peripheral </span><span lang="EN-CA" mso-bidi-language:="" new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">blood PRP </span><span new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">preparations regarding cell proliferation and migration, but the difference to 10% FBS was significant. </span><span lang="EN-CA" mso-bidi-language:="" new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">1% and 50% PRP reduced cellular proliferation. The 20% umbilical </span><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">cord</span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height: 200%;font-family:" times=""> blood</span><span lang="EN-CA" new="" style="font-size:12.0pt;line-height:200%;font-family:" times=""> </span><span lang="EN-CA" mso-bidi-language:="" new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">PRP and 10% adult </span><span new="" style="font-size:12.0pt;line-height:200%; font-family:" times="">peripheral </span><span lang="EN-CA" mso-fareast-language:="" new="" style="font-size:12.0pt;line-height:200%;font-family: " times="">blood PRP had a significant stimulatory effect on the migration of the skin fibroblast cells in comparison with 10% FBS. </span></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><b><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">CONCLUSION</span></b></p> <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:200%;direction:ltr;unicode-bidi:embed"><span new="" style="font-size:12.0pt;line-height:200%;font-family:" times="">As PRP could promote the migration and proliferation of dermal fibroblasts, it can be safely added in cultures when treatment of chronic wounds without triggering the immune response is needed.</span></p> Umbilical cord blood, Platelet rich plasma, Proliferation, Migration, Skin fibroblast 198 205 http://wjps.ir/browse.php?a_code=A-10-104-5&slc_lang=en&sid=1 Seyedeh-Sara Hashemi 10031947532846003604 10031947532846003604 No Mahdokht Mahmoodi 10031947532846003605 10031947532846003605 No Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Ali Reza Rafati 10031947532846003606 10031947532846003606 Yes Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran Davood Mehrabani 10031947532846003607 10031947532846003607 No Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran