fa تخصصي Special مقالات اصيل Original Article <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><b><span new="" style="font-size:12.0pt;line-height:200%;mso-bidi-font-family:" times="">BACKGROUND</span></b></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><span new="" style="font-size:12.0pt;line-height:200%;mso-bidi-font-family:" times="">Differences in causes, severities, areas of stenosis, and the association with swallowing and phonation of larynx and trachea can result into Laryngotracheal stenosis (LTS).<span class="hps">This study evaluated the healing effect of bone marrow stem cells (BMSCs) in experimentally induced </span>LTS<span class="hps"> dog model.</span></span></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><b><span new="" style="font-size:12.0pt;line-height:200%;mso-bidi-font-family:" times="">METHODS</span></b></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><span style=&quot;font-size:12.0pt;line-height:200%;mso-ascii-font-family: &quot; times=&quot;&quot; new=&quot;&quot; roman&quot;;mso-ascii-theme-font:major-bidi;mso-hansi-font-family:&quot;times=&quot;&quot; roman&quot;;=&quot;&quot; mso-hansi-theme-font:major-bidi;mso-bidi-font-family:&quot;times=&quot;&quot; mso-bidi-theme-font:major-bidi&quot;=&quot;&quot;>Seven dogs were enrolled. <span style="font-size:12.0pt;line-height:200%">BMSCs were isolated from proximal humerus and shoulder of a dog and cultured in media containing alpha minimal essential medium, fetal bovine serum, penicillin and streptomycin, and L-glutamine. BMSCs were characterized morphologically, by RT-PCR, and osteogenic induction. Karyotyping was undertaken for chromosomal stability. <span class="hps">Mechanical trauma to laryngeal mucosa </span>was identically <span class="hps">conducted by</span> Tru-cut punch forceps in right and left vocal folds. Two milliliter of conditioned media or BMSCs (2</span><span new="" style="font-size:12.0pt;line-height:200%; mso-bidi-font-family:" times="">&times;</span><span style="font-size:12.0pt; line-height:200%">10⁶) were injected in the right site of the tissue and the left side was considered as control after LTS induction. The larynx was visualized 2, 4 and 6 weeks after treatment. <span class="hps">Six weeks post-treatment, the larynges were evaluated histologically. </span></span></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><b><span new="" style="font-size:12.0pt;line-height:200%;mso-bidi-font-family:" times="">RESULTS</span></b></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><span style="font-size:12.0pt;line-height:200%">BMSCs were adhered to culture flasks, spindle shape and positive for mesenchymal marker and negative for hematopoietic markers. Osteogenic induction was verified by Alizarin red staining. Karyotype was normal. </span><span new="" style="font-size: 12.0pt;line-height:200%;mso-bidi-font-family:" times="">A complete epithelialization and minimal chronic inflammatory cell infiltration were noted in submucosa of both left (control) and right (cases) vocal folds. The healing effect of conditioned media and BMSCs in comparison to the control group was more prominent. </span></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><b><span new="" style="font-size:12.0pt;line-height:200%;mso-bidi-font-family:" times="">CONCLUSION</span></b></p> <p class="MsoNormal" style="line-height: 200%; direction: ltr; unicode-bidi: embed;"><span new="" style="font-size:12.0pt;line-height:200%;mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin;mso-bidi-font-family:" times="">As thickness of fibrosis in cases were less than control group, conditioned media and BMSCs were shown to be good choices in healing of LTS.</span></p> Healing, Conditioned media, Bone marrow, Stem cell, Laryngotracheal stenosis, Dog 190 197 http://wjps.ir/browse.php?a_code=A-10-1-65&slc_lang=fa&sid=1 Kamyar Iravani 10031947532846003598 10031947532846003598 No ENT Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran Arash Sobhanmanesh 10031947532846003599 10031947532846003599 No ENT Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran Mohammad Javad Ashraf 10031947532846003600 10031947532846003600 No Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Seyed Basir Hashemi 10031947532846003601 10031947532846003601 No ENT Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran Davood Mehrabani 10031947532846003602 10031947532846003602 Yes Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Shahrokh Zare 10031947532846003603 10031947532846003603 No Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran