en عمومى General مقالات اصيل Original Article BACKGROUND<br> Collagen is the main product in pharmaceutics and food industry with a high demand. Collagen can be extracted from several tissues such as skin, bone and tendon, etc. Collagen can be used in tissue engineering researches as a substrate of wound healing and nerve regeneration. Extraction methods of collagen are various with different purities. In this research, we aimed to extract collagen from human placenta with a modified method.&nbsp;<br> METHODS<br> This modified approach was used for extracting of collagen from human placenta with acetic acid and NaCl treatment using different concentrations.&nbsp;<br> RESULTS<br> SDS page showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. There was no significant difference between extracted collagen from human placenta and standard collagen in western blot analysis.&nbsp;<br> CONCLUSION<br> It was concluded that human placenta can be an alternative source of collagen with high purity for biomedical applications such as tissue engineering, stem cell therapy and research. Collagen, Huma, Placenta, Extraction, Characterization 352 358 http://wjps.ir/browse.php?a_code=A-10-443-1&slc_lang=en&sid=1 Ali Karami karami.ali2001@gmail.com 10031947532846007306 10031947532846007306 No Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Hamid Tebyanian tebyan.hamid@yahoo.com 10031947532846007307 10031947532846007307 Yes Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran Reza Sayyad Soufdoost 10031947532846007308 10031947532846007308 No Imam Khomeini Clinic of Dentistry, Tehran, Iran Ebrahim Motavallian motavallian.e@gmail.com 10031947532846007309 10031947532846007309 No Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran Aref Barkhordari barkhordariarefe@gmail.com 10031947532846007310 10031947532846007310 No Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Mohammad Reza Nourani nouranim82@gmail.com 10031947532846007311 10031947532846007311 No Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;