WORLD JOURNAL OF PLASTIC SURGERY
مجله جهانی جراحی پلاستیک
WJPS
Medical Sciences
http://wjps.ir
1
admin
2228-7914
2252-0724
8
10.61186/wjps
14
8888
13
en
jalali
1399
11
1
gregorian
2021
2
1
10
2
online
1
fulltext
en
Evaluation of Acellular Dermal Matrix (ADM) as a Scaf-fold for Adipose-Derived Stem Cell Transfer in the Rat Model
تخصصي
Special
مقالات اصيل
Original Article
BACKGROUND<br>
This study was designed for the evaluation of Acellular Dermal Matrix (ADM) as a scaffold for adipose-derived stem cell transferring in the rat model.<br>
METHODS<br>
This experimental study was done in the Burn Research Center of Iran University of Medical Sciences and Bonyakhteh Research Center, Tehran, Iran according to the standards of laboratory animals. Overall, 26 healthy Sprague-Dawley rats were used. Two of them were used to prepare ADM. In group one, the first wound on each, rat was spread with the mixture of fibrin gel and autologous stem cell. Only the stem cells combined with fibrinogen were spread on the other wound. In group two, the first wound on each rat was covered only with ADM, and the second wound was covered with gauze Vaseline. To perform sampling we used observation and photography at 7-30 days. Overall, 48 samples were taken of all the rats using skin punch biopsy on the 30th day for histopathology evaluation.<br>
RESULTS<br>
There were significant differences in each group; however, the difference between different groups on days was not significant. In pathology, epithelialization, vascularization, the amount of collagen, collagen arrangement, the number of fibroblasts, and inflammation indices were investigated. The total score in each group was used for analysis. In statistical analysis, there was no pathology score difference among groups.<br>
CONCLUSION<br>
Using stem cells with or without ADM could not enhance the process of wound healing or improve pathology indices.
Stem cell, Tissue engineering, Acellular Dermis
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http://wjps.ir/browse.php?a_code=A-10-270-3&slc_lang=en&sid=1
Maryam
Jahanian
100319475328460010608
100319475328460010608
No
Burn Research Center, Hazrat Fatima Hospital, Rehabilitation Research Center, School of Rehabilitation Sci-ences, Iran University of Medical Sciences, Tehran, Iran.
Sara
Hoseini
100319475328460010609
100319475328460010609
No
Burn Research Center, Rajaie Cardi-ovascular Medical and Research Cen-ter, Iran University of Medical Sci-ences, Tehran, Iran
Amir
Atashi
100319475328460010610
100319475328460010610
No
Stem Cell and Tissue Engineering Research Center, Shahroud Universi-ty of Medical Sciences, Shahroud, Iran.
Mohsen
Saberi
100319475328460010611
100319475328460010611
No
Community Medicine, Quran and Hadis Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences Tehran, Iran.
Seyyed Aboozar
Hoseini
100319475328460010612
100319475328460010612
No
Burn Research Center, Hazrat Fatima Hospital, Iran University of Medical Sciences, Tehran, Iran.
Kambiz
Mozaffari
100319475328460010613
100319475328460010613
No
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mohammad Javad
Fatemi
mjfatemi41@gmil.com
100319475328460010614
100319475328460010614
Yes
Department of Plastic & Reconstruc-tive Surgery, Burn Research Center, Hazrat Fatima Hospital Iran Universi-ty of Medical Sciences, Tehran, Iran.