en تخصصي Special مقالات اصيل Original Article <strong>BACKGROUND</strong><br> Wound healing is a critical clinical concept. We aimed to evaluate the effects of topical <em>Mentha piperita</em> essence on cutaneous wound healing.<br> <strong>METHODS</strong><br> This randomized controlled trial was conducted in Tehran University of Medical Sciences, Tehran, Iran in 2019. Square-shaped 1.5&times;1.5 cm wounds were made on the neck of 60 male Wistar rats in a sterile condition. Samples were randomly divided into a control group and three experimental groups. Group A treated with <em>M. piperita</em> essence and Vaseline. The second group received the <em>M. piperita</em> essence, and the third group received Vaseline. Histological specimens were obtained in 4^th, 7^th, and 14^th days and were explored for fibroblasts, epithelial cells, inflammatory cells, and vessels. RT-PCR was performed for molecular and gene expression evaluation of TGF-&beta;.<br> <strong>RESULTS</strong><br> <a name="_Hlk29106962">The <em>M. piperita</em></a> essence increases <a name="_Hlk28958669"></a>TGF-&beta; gene expression as an important factor in wound healing. After 14 d, group A, who received <em>M. piperita</em> and Vaseline, had 99.73% of wound healing with the mean wound size of 0.006 cm² while wound healing in the control group was only 52%. Samples treated with <em>M. piperita</em> have 74.58% wound healing following by group treated with Vaseline, which was 67.02% (<em>P</em><0.05), respectively.<br> <strong>CONCLUSION</strong><br> The application of the <em>M. piperita</em> essence for wound healing accelerates the process and improves outcomes. Mentha piperita, Peppermint, Wound healing, Herbal medicine, TGF- β 86 96 http://wjps.ir/browse.php?a_code=A-10-570-2&slc_lang=en&sid=1 Mohammad Hosein Amirzade-Iranaq 100319475328460012484 100319475328460012484 Yes 1. Universal Network of Interdisciplinary Research in Oral and Maxillofacial Surgery (UNIROMS), Universal Scientific Education and Research Network (USERN), Tehran, Iran Maryam Tajik 100319475328460012485 100319475328460012485 No 2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mina Amirmohammadi 100319475328460012486 100319475328460012486 No 2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Farzane Rezaei Yazdi 100319475328460012487 100319475328460012487 No 2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Nasrin Takzaree takzaree@gmail.com 100319475328460012488 100319475328460012488 No 5. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran