Volume 10, Issue 2 (2-2021)                   WJPS 2021, 10(2): 82-88 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Omranifard M, Rasti Ardakani M, Abdali H, Mortazavi P, Hoseini S, Hoghoughi M A. Comparative Experimental Study of Dermal Stability: Acellular Dermal Matrix versus Crayopreserved Dermis. WJPS. 2021; 10 (2) :82-88
URL: http://wjps.ir/article-1-765-en.html
Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran
Abstract:   (321 Views)
BACKGROUND
Given the potential usefulness of Acellular Dermal Matrices (ADM) for wound healing, we aimed to evaluate the stability, histological characteristics, and effectiveness of ADM compared with cryopreserved dermis (CPD) in rat models.
METHODS
This experimental study was conducted in the Department of Surgery, Isfahan University of Medical Sciences, Isfahan, Iran, from January to March 2015. The prepared ADM and CPD were transplanted to the full-thickness skin defects on the back of Sprague-Dawley rats. Forty-five days after grafting, the tissues were harvested for histological examination. These two types of the dermis' quality and stability were compared with consideration of the following factors; inflammation, fibroblasts migration, vascularization, collagen formation, capsule formation, and microabscess formation.
RESULTS
From 19 selected rates, nine received CPD, and ten were treated with ADM. After transplantation, the mean (SD) weight of ADM and CPD grafts were 1.74 (0.07) and 1.45 (0.77), respectively (P<0.001). The frequency of inflammation was significantly higher in CPD grafts (P<0.01). Higher grades of collagen organization, fibroblast spreading, and vascularization were more frequent in ADM grafts (P<0.01). The frequency of capsule and microabscesses formation was not significantly different between studied groups.
CONCLUSION
ADM have a superior effect than CPD in the wound healing process. Both samples had a similar effect in capsule and microabscesses formation and higher costs of ADM preparation. According to the physicians' decision and evaluation of the process's cost-effectiveness, CPD could be appropriately used as an alternative to ADM.
Full-Text [PDF 656 kb]   (129 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2021/04/20 | Accepted: 2021/02/22 | Published: 2021/06/25

References
1. Shevchenko RV, James SL, James SE. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface. 2010; 7(43):229-58. [DOI:10.1098/rsif.2009.0403]
2. Bloemen MCT, van Leeuwen MCE, van Vucht NE, van Zuijlen PPM, Middelkoop E. Dermal substitution in acute burns and reconstructive surgery: a 12-year follow-up. Plast Reconstr Surg. 2010; 125 (5):1450-9. [DOI:10.1097/PRS.0b013e3181d62b08]
3. Clark RA, Ghosh K, Tonnesen MG. Tissue engineering for cutaneous wounds. J Invest Dermatol. 2007;127:1018-1029. [DOI:10.1038/sj.jid.5700715]
4. Campitiello E, Della Corte A, Fattopace A, D'Acunzi D, Canonico S. The use of artificial dermis in the treatment of chronic and acute wounds: Regeneration of dermis and wound healing. Acta Biomed. 2005;76(Suppl 1):69-71.
5. Melman L, Jenkins ED, Hamilton NA, Bender LC, Brodt MD, Deeken CR, Greco SC, Frisella MM, Matthews BD. Early biocompatibility of crosslinked and non-crosslinked biologic meshes in a porcine model of ventral hernia repair. Hernia. 2011 ;15(2):157-64. [DOI:10.1007/s10029-010-0770-0]
6. Debels H, Hamdi M, Abberton K, Morrison W. Dermal matrices and bioengineered skin substitutes: a critical review of current options. Plast Reconstr Surg Glob Open. 2015 ;3(1):e284. [DOI:10.1097/GOX.0000000000000219]
7. Truong AT, Kowal-Vern A, Latenser BA, Wiley DE, Walter RJ. Comparison of dermal substitutes in wound healing utilizing a nude mouse model. J Burns Wounds. 2005;4:e4.
8. Juhasz I, Kiss B, Lukacs L, Erdei I, Peter Z, Remenyik E. Long-term followup of dermal substitution with acellular dermal implant in burns and postburn scar corrections. Dermatol Res Pract. 2010;2010:210150. [DOI:10.1155/2010/210150]
9. Johnson RK, Wright CK, Gandhi A, Charny MC, Barr L. Cost minimisation analysis of using acellular dermal matrix (Strattice™) for breast reconstruction compared with standard techniques. Eur J Surg Oncol. 2013 ;39(3):242-7. [DOI:10.1016/j.ejso.2012.12.002]
10. Krishnan NM, Chatterjee A, Rosenkranz KM, Powell SG, Nigriny JF, Vidal DC. The cost effectiveness of acellular dermal matrix in expander-implant immediate breast reconstruction. J Plast Reconstr Aesthet Surg. 2014;67(4):468-76. [DOI:10.1016/j.bjps.2013.12.035]
11. Lee LF, Porch JV, Spenler W, Garner WL. Integra in lower extremity reconstruction after burn injury. Plast Reconstr Surg. 2008;121:1256-62. [DOI:10.1097/01.prs.0000304237.54236.66]
12. Tsoutsos D, Stratigos A, Gravvanis A, Zapandioti P, Kakagia D. Burned breast reconstruction by expanded artificial dermal substitute. J Burn Care Res. 2007;28:530-2. [DOI:10.1097/BCR.0B013E318053DAC5]
13. Burd A, Wong PS. One-stage Integra reconstruction in head and neck defects. J Plast Reconstr Aesthet Surg. 2010;63:404-9. [DOI:10.1016/j.bjps.2008.11.105]
14. Chalmers RL, Smock E, Geh JL. Experience of Integra(®) in cancer reconstructive surgery. J Plast Reconstr Aesthet Surg. 2010;63:2081-90. [DOI:10.1016/j.bjps.2010.02.025]
15. Rindler R, Garcia C. Letter: The use of a dermal substitute and thin skin grafts in the cure of "complex" leg ulcers. Dermatol Surg. 2010;36:426. [DOI:10.1111/j.1524-4725.2009.01461.x]
16. Sato Y, Yan Ni Yang DZP Su Hua Wu, Xin Zhou, Jing Liu, Zeng Xue Dong. Comparison of method for preparing xenogeneic acellular dermal matrix and significance in transplantation. Chinese Journal of Clinical Rehabilitation. 2004;8(23):4772-4773.
17. You C, Wang X, Zheng Y, Han C. Three types of dermal grafts in rats: the importance of mechanical property and structural design. Biomed Eng Online. 2013 ;12:125. [DOI:10.1186/1475-925X-12-125]
18. Ma ZF, Chai JK, Yang HM, Liu Q, Xu MH, Yin HN. Acellular porcine dermal matrix produced with different methods and an experimental study on its transplantation to skin wound. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005 ;17(2):92-4.
19. Purdue GF, Hunt JL, Still JM Jr, Law EJ, Herndon DN, Goldfarb IW, et al. A multicenter clinical trial of a biosynthetic skin replacement, Dermagraft-TC, compared with cryopreserved human cadaver skin for temporary coverage of excised burn wounds. J Burn Care Rehabil. 1997;18(1, Part 1):52-57. [DOI:10.1097/00004630-199701000-00009]
20. Mizuno H, Takeda A, Uchinuma E. Creation of an acellular dermal matrix from frozen skin. Aesthetic Plast Surg. 1999 ;23(5):316-22. [DOI:10.1007/s002669900292]
21. Brosious JP, Wong N, Fowler G, Stephenson LL, Wang WZ, Zamboni WA, Taghipour-Khiabani K. Evaluation of AlloMax acellular dermal matrix for objective collagen deposition. J Reconstr Microsurg. 2014;30(1):31-4. [DOI:10.1055/s-0033-1349721]
22. Lugo LM, Lei P, Andreadis ST. Vascularization of the dermal support enhances wound re-epithelialization by in situ delivery of epidermal keratinocytes. Tissue Eng Part A. 2011 ;17(5-6):665-75. [DOI:10.1089/ten.tea.2010.0125]
23. Gopal J, Pauli EM, Haluck RS, Moyer MT, Mathew A. Intramural acellular porcine dermal matrix (APDM)-assisted gastrotomy closure for natural orifice transluminal endoscopic surgery (NOTES). Surg Endosc. 2012 ;26(8):2322-30. [DOI:10.1007/s00464-012-2183-x]
24. Stump A, Holton LH 3rd, Connor J, Harper JR, Slezak S, Silverman RP. The use of acellular dermal matrix to prevent capsule formation around implants in a primate model. Plast Reconstr Surg. 2009 ;124(1):82-91. [DOI:10.1097/PRS.0b013e3181ab112d]
25. Basu CB, Leong M, Hicks J. Acellular cadaveric dermis decreases the inflammatory response in capsule formation in reconstructive breast surgery. Plast Reconstr Surg. 2010;126:1842-7. [DOI:10.1097/PRS.0b013e3181f44674]
26. Eming SA, Krieg T, Davidson JM. Inflammation in wound repair: molecular and cellular mechanisms. J Investig Dermatol Symp Proc. 2007; 127(3):514-25. [DOI:10.1038/sj.jid.5700701]
27. Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008; 453(7193):314-21. [DOI:10.1038/nature07039]

Add your comments about this article : Your username or Email:
CAPTCHA

© 2021 CC BY-NC 4.0 | World Journal of Plastic Surgery

Designed & Developed by : Yektaweb