WORLD JOURNAL OF PLASTIC SURGERY
Fri, May 23, 2025
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Volume 14, Issue 1 (2025)
WJPS 2025, 14(1): 33-42 |
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Ganjipour A, Nasiri-Formi E, Azizi S, Akbari J, Akbari H, Hashemi S M H. The Effect of Zataria multiflora Nanoemulsion Gel on Rat Surgical Wound Healing. WJPS 2025; 14 (1) :33-42
URL: http://wjps.ir/article-1-1371-en.html
URL: http://wjps.ir/article-1-1371-en.html
Alireza Ganjipour *1 
, Ebrahim Nasiri-Formi2 , Soheil Azizi3 , Jafar Akbari4 , Hooshang Akbari , Seyyed Mohammad Hassan Hashemi5
, Ebrahim Nasiri-Formi2 , Soheil Azizi3 , Jafar Akbari4 , Hooshang Akbari , Seyyed Mohammad Hassan Hashemi5
1- Department of Anesthesiology, Operating room, School Allied medical Sciences,, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran & 2. Department of Clinical Sciences, School of Medical Sciences, Khomein Faculty of Medical Sciences, Khomein,Iran
2- 3. Department of Anesthesiology, Operating room, School of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran & 4. Department of Persian Medicine, Faculty of Traditional and Complementary Medicine Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Laboratory Sciences, School of Allied Medical Science, Mazandaran University of Medical Sciences, Sari, Iran
3- 5. Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4- 6. Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
5- Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2- 3. Department of Anesthesiology, Operating room, School of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran & 4. Department of Persian Medicine, Faculty of Traditional and Complementary Medicine Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Laboratory Sciences, School of Allied Medical Science, Mazandaran University of Medical Sciences, Sari, Iran
3- 5. Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4- 6. Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
5- Department of Pharmaceutics, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Abstract: (580 Views)
Background: We aimed to determine the effect of Zataria multiflora Nanoemulsion gel on surgical wound healing.
Methods: This experimental study was conducted in the years 2021-2022 at the Animal Research Center of Mazandaran University of Medical Sciences, northern Iran. Forty two male Westar rats were randomly divided into six groups (n=7). After a surgical incision of full thickness with a 3 cm diameter, they were treated for 21 days with diltiazem 2% (positive control), placebo, and Z. multiflora emulsions and nanoemulsions at 2% and 4%, respectively. Macroscopic parameters of wound area and contraction, as well as pathological factors such as granulation, angiogenesis, epithelialization, collagen organization, bacterial colony, inflammation, creatine and epidermal thickness, hair follicles, and lymphatic ducts, were examined.
Results: The mean wound size and contraction of the placebo group differed significantly from the other groups on all days, and on some days, the results indicated more favorable effects of nanoemulsions than Diltiazem. Based on the microscopic findings, the average scores on the seventh day were nearly different (P= 0.051); however, all groups scored higher than the placebo group. On the 21st day, the best results were related to the 4% Nanoemulsion (242.5), 2% Nanoemulsion (159.4), and 4% emulsion (159.3), followed by diltiazem (154.60), 2% emulsion (146.5), and placebo (70.7).
Conclusion: Z. multiflora emulsions and nanoemulsions at 2% and 4% could be effective in healing surgical wounds, and the use of 4% Nanoemulsion yields the best results. This is recommended for use in clinical trial studies.
Methods: This experimental study was conducted in the years 2021-2022 at the Animal Research Center of Mazandaran University of Medical Sciences, northern Iran. Forty two male Westar rats were randomly divided into six groups (n=7). After a surgical incision of full thickness with a 3 cm diameter, they were treated for 21 days with diltiazem 2% (positive control), placebo, and Z. multiflora emulsions and nanoemulsions at 2% and 4%, respectively. Macroscopic parameters of wound area and contraction, as well as pathological factors such as granulation, angiogenesis, epithelialization, collagen organization, bacterial colony, inflammation, creatine and epidermal thickness, hair follicles, and lymphatic ducts, were examined.
Results: The mean wound size and contraction of the placebo group differed significantly from the other groups on all days, and on some days, the results indicated more favorable effects of nanoemulsions than Diltiazem. Based on the microscopic findings, the average scores on the seventh day were nearly different (P= 0.051); however, all groups scored higher than the placebo group. On the 21st day, the best results were related to the 4% Nanoemulsion (242.5), 2% Nanoemulsion (159.4), and 4% emulsion (159.3), followed by diltiazem (154.60), 2% emulsion (146.5), and placebo (70.7).
Conclusion: Z. multiflora emulsions and nanoemulsions at 2% and 4% could be effective in healing surgical wounds, and the use of 4% Nanoemulsion yields the best results. This is recommended for use in clinical trial studies.
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