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Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study
Honnegowda Thittamaranahalli Muguregowda , Pramod K Kumar , Padmanabha Udupa E
Kasturba Medical College, Manipal, Karnataka, India
Abstract:   (38 Views)

BACKGROUND
Malondialdehyde (MDA) is an oxidant cause damage to membranes, DNA, proteins, and lipids at the cellular level. Antioxidants minimize the effects of oxidants and thus helps in formation of healthy granulation tissue with higher level of hydroxyproline, total protein. This study compared the effect of limited access dressing (LAD) vs. conventional closed dressing biochemically and histopathologically.
METHODS
Seventy-two burn 12-65 years old patients with mean wound size of 14 cm2 were divided to two groups of LAD (n=37), and conventional dressing groups (n=35). Various biochemical parameters were measured in granulation tissue.  Histopathological analysis of the granulation tissue was studied too.
RESULTS
LAD group showed significant increase in hydroxyproline, total protein, GSH, and GPx and decrease in MDA levels compared to conventional dressing group. A significant negative correlation between GSH and MDA was noted in LAD group, but in conventional dressing group there was no significant correlation. A significant negative correlation between GPx and MDA was noticed in LAD group, but in conventional dressing group was not significant. There was a histologically fewer inflammatory cells, increased and well organized extracellular matrix deposit, more angiogenesis in LAD group after 10 days while the difference was significant between the groups.
CONCLUSION
Our study showed a significant reduction in oxidative stress biomarker of MDA, increase in hydroxyproline, total protein, antioxidants and amount of ECM deposition, number of blood vessels and a decrease in the amount of inflammatory cells and necrotic tissue in LAD group indicating the better healing effect of burn wounds. 

Keywords: Burn, Wound, ROS, Limited access dressing, Malondialdhyde, Antioxidant
Full-Text [PDF 3165 kb]   (16 Downloads)    
Type of Study: Original Article | Subject: Special
Received: 2016/10/14 | Accepted: 2018/01/7 | Published: 2018/01/7
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Thittamaranahalli Muguregowda H, Kumar P K, E P U. Wound Healing Potential of Intermittent Negative Pressure under Limited Access Dressing in Burn Patients: Biochemical and Histopathological Study . wjps. 2018; 7 (1)
URL: http://wjps.ir/article-1-291-en.html
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