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Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications
Ali Karami, Hamid Tebyanian , Reza Sayyad Soufdoost, Ebrahim Motavallian, Aref Barkhordari, Mohammad Reza Nourani
Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (103 Views)
BACKGROUND
Collagen is the main product in pharmaceutics and food industry with a high demand. Collagen can be extracted from several tissues such as skin, bone and tendon, etc. Collagen can be used in tissue engineering researches as a substrate of wound healing and nerve regeneration. Extraction methods of collagen are various with different purities. In this research, we aimed to extract collagen from human placenta with a modified method. 
METHODS
This modified approach was used for extracting of collagen from human placenta with acetic acid and NaCl treatment using different concentrations. 
RESULTS
SDS page showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. There was no significant difference between extracted collagen from human placenta and standard collagen in western blot analysis. 
CONCLUSION
It was concluded that human placenta can be an alternative source of collagen with high purity for biomedical applications such as tissue engineering, stem cell therapy and research.
Keywords: Collagen, Huma, Placenta, Extraction, Characterization
Full-Text [PDF 944 kb]   (38 Downloads)    
Type of Study: Original Article | Subject: General
Received: 2019/02/11
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مجله جهانی جراحی پلاستیک World Journal of Plastic Surgery
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